BDPFLNHSesterisanadvanceddyefor488nmchannel,areplacementforfluorescein,amoleculeidenticaltoBODIPYFL®NHSester.Anamino-reactivedyeforthelabelingofproteinsandpeptides.
WhiletheabsorbanceandemissionspectraofthismoleculestaywithinFAMexcitationandemissionchannels,thisdyeprovidesmuchbetterphotostABIlity,andoutstandingbrightness.ThefluorescencespectrumofBODIPY-FLisnarrowerthanthatofFAM.Thisprovidesabetterbrightnessformonochromatorbasedinstruments,whenemissionwavelengthcanbetunedtodyemaximum.
Thedyeisneutral,possesseslowmolecularweight,andretainshighquantumyieldinconjugates.
Thedyeisagoodreplacementforfluorescein(FAM),BODIPY-FL,AlexaFluor488,DyLight488,Cy2,andother488nmdyes.
Appearance: | orangesolid |
MassspecM+increment: | 274.1 |
Molecularweight: | 389.16 |
Molecularformula: | C18H18BF2N3O4 |
Solubility: | Goodinorganicsolvents(DMF,DMSO,dichlromethane),limitedinwater |
Qualitycontrol: | NMR1H,HPLC-MS(95+%) |
Storageconditions: | Storage:12monthsafterreceivalat-20°Cinthedark.Transportation:atroomtemperatureforupto3weeks.Avoidprolongedexposuretolight.Desiccate. |
Excitationmaximum,nm: | 503 |
ε,L⋅mol−1⋅cm−1: | 80000 |
Emissionmaximum,nm: | 509 |
Fluorescencequantumyield: | 0.97 |
CF260: | 0.015 |
CF280: | 0.027 |
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