With its quantum yield approaching unity, ROX has become a popular dye for qPCR and microscopy. This bright fluorophore is available as an alkyne for copper-catalyzed Click chemistry reaction.
This is a pure 5-isomer of ROX.
ROX is prone to oxidation. Therefore, its derivatives should be preferably stored in inert atmosphere.
Appearance: | purple solid |
Mass spec M+ increment: | 571.3 |
Molecular weight: | 571.66 |
Molecular formula: | C36H33N3O4 |
Solubility: | good in DMSO, DMF, methanol, ethanol |
Quality control: | NMR 1H, HPLC-MS (95%) |
Storage conditions: | Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
Excitation maximum, nm: | 570 |
ε, L⋅mol−1⋅cm−1: | 80000 |
Emission maximum, nm: | 591 |
Fluorescence quantum yield: | 1.00 |
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