Sulfo-Cyanine5.5dyeisawatersolublefarredtoNIRemittingdyewhichisveryhydrophilicduetothepresenceoffoursulfogroups.Sulfo-Cyanine5.5derivativesexhibithighwatersolubility.Thedyeisaperfectchoiceforthelabelingofsensitiveproteins,nanoparticles,andhighlyhydrophylicbiopolymers.
Thismaleimideisathiolreactivedyethatselectivelylabelscysteineresidues.Disulfidebondsofnativeproteinsshouldbereducedwithanappropriatereducingagent,suchasTCEP,accordingtoourrecommendedprotocol.
Appearance: | darkcoloredsolid |
MassspecM+increment: | 1024.2 |
Molecularweight: | 1139.43 |
Molecularformula: | C46H45K3N4O15S4 |
Solubility: | goodinwater,DMSO,DMF |
Qualitycontrol: | NMR1H,HPLC-MS(95%) |
Storageconditions: | Storage:12monthsafterreceivalat-20°Cinthedark.Transportation:atroomtemperatureforupto3weeks.Avoidprolongedexposuretolight.Desiccate. |
Excitationmaximum,nm: | 673 |
ε,L⋅mol−1⋅cm−1: | 195000 |
Emissionmaximum,nm: | 691 |
CF260: | 0.09 |
CF280: | 0.11 |
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