Cyanine5hydrazideisareactivedyeforthelabelingofaldehydesandketones,ananalogofCy5®hydrazide.
Thisdyereactssmoothlyandnearlyquantitativelywithvariouscarbonylgroupsencounteredinbiomolecules.Examplesareproteinssubjectedtooxidativestress,glycosylatedproteinspre-activatedbyperiodateoxidation(includingantobodies),andoligonucleotideswithaldehydemoieties.
Cyanine5hydrazidereplacescarbonyl-reactiveCy5®,AlexaFluor647,DyLight649dyes.
Appearance: | darkbluepowder |
Molecularweight: | 569.61 |
Molecularformula: | C32H42Cl2N4O |
Solubility: | moderatesolubilityinwater,goodinpolarorganicsolvents(DMF,DMSO,alcohols) |
Qualitycontrol: | NMR1H,HPLC-MS(95%) |
Storageconditions: | Storage:24monthsafterreceivalat-20°Cinthedark.Transportation:atroomtemperatureforupto3weeks.Avoidprolongedexposuretolight.Desiccate. |
Excitationmaximum,nm: | 646 |
ε,L⋅mol−1⋅cm−1: | 250000 |
Emissionmaximum,nm: | 662 |
Fluorescencequantumyield: | 0.2 |
CF260: | 0.03 |
CF280: | 0.04 |
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