Pyrene hydrazide is a carbonyl reactive dye for the attachment of pyrene polycyclic aromatic hydrocarbon residues to aldehydes and ketones.
Pyrene is a blue-emitting fluorescent label which is especially useful as a proximity probe. When two pyrene residues are located close to each other, a longer-wavelength excimer fluorescence can be detected. Pyrene can also be FRET donor to other fluorophores. Its excited state possesses an unusually long lifetime of more than 100 ns, which allows to use this fluorophore in time-gated experiments.
ChemDraw format
General properties
| Appearance: | off white solid |
| Mass spec M+ increment: | 284.1 |
| Molecular weight: | 338.83 |
| Molecular formula: | C20H19ClN2O |
| IUPAC name: | 4-(1-pyrenyl)butyric acid hydrazide hydrochloride |
| Quality control: | NMR 1H, HPLC-MS (95%) |
| Storage conditions: | Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
Spectral properties
| Excitation maximum, nm: | 343; 326; 313; 276; 265; 242; 234 |
| Emission maximum, nm: | 377; 397 |
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