Tetrazine-trans-cycloocteneligationisamongthefastestbioconjugationreactionsknowntodate.
Thiswatersolublesulfo-Cyanine3derivativecontainsmethyltetrazinemoietyforthecouplingwithtrans-cyclooctenes.MethyltetrazinespossessoptimalstABIlityatphysiologicalpH,whilemaintainingextremelyhighreactivitytowardscyclooctenes.
Thefluorophoreisbright,photostable,andisalsoverywellvisIBLewithnakedeye.
Appearance: | redpowder |
Molecularweight: | 838.05 |
Molecularformula: | C40H44KN7O7S2 |
IUPACname: | Potassium(E)-2-[(E)-3-{3,3-Dimethyl-1-[6-({[p-(6-methyl-1,2,4,5-tetrazin-3-yl)phenyl]methyl}amino)-6-oxohexyl]-5-(oxysulfonyl)-3H-indol-2-yl}-2-propenylidene]-1-methyl-3,3-dimethyl-5-indolinesulfonate |
Solubility: | goodinwater,DMF,DMSO |
Qualitycontrol: | NMR1H,HPLC-MS(95%) |
Storageconditions: | Storage:24monthsafterreceivalat-20°Cinthedark.Transportation:atroomtemperatureforupto3weeks.Avoidprolongedexposuretolight.Desiccate. |
Excitationmaximum,nm: | 548 |
ε,L⋅mol−1⋅cm−1: | 162000 |
Emissionmaximum,nm: | 563 |
Fluorescencequantumyield: | 0.1 |
CF260: | 0.03 |
CF280: | 0.06 |
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