Phosphoramiditeforthesynthesisofoligonucleotideswith5"-terminalalkyneforClickchemistry.
Thisalkyneamiditehasseveraladvantagesover5’-hexynylphosphoramidite,5"-butynyl-CEP,andother5"-terminalalkynephosphoramidites.First,itissolidcompoundwhichiseasiertohandleanddispense.Andduetoitsstructure,itisalsomorestableinsolution,andhaslongershelflife
Diluentforthisphosphoramiditeisacetonitrile,5mincouplingtimeisrecommended.Becausethisamiditedoesnotcontain5"-terminalDMTgroup,no5"-deprotectionneeded.Oligonucleotidesshouldbedeblockedunderstandardconditions,andpurifiedbyPAGE,orionexchangeHPLC.
OligonucleotideswiththismodificationareidealfortheuseinClickChemistry(seeourrecommendedprotocol).
Appearance: | colorlesssolid |
Molecularweight: | 409.50 |
Molecularformula: | C21H36N3O3P |
IUPACname: | trans-4-(5-Hexynoylamino)cyclohexyloxy-N,N-diisopropylamino-2-cyanoethyloxyphosphine |
Solubility: | goodinacetonitrileanddichloromethane |
Qualitycontrol: | NMR1H(95%)and31P,HPLC-MS |
Storageconditions: | Storage:24monthsafterreceivalat-20°C.Transportation:atroomtemperatureforupto3weeks.Desiccate. |
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